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KMID : 0861020120270010059
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Korea Journal of Herbology
2012 Volume.27 No. 1 p.59 ~ p.64
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Effect of Forsythiae Fructus ethanol extract on inflammatory cytokine production and cellular signaling pathways in mouse macrophages
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Nam Jung-Bum
Lee Mi-Hwa
Choi Ho-Young
Sohn Nak-Won
Kang Hee
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Abstract
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Objective: This study was performed to evaluate the effect of Forsythiae Fructus (FF) ethanol extract on
inflammatory cytokine production and its underlying mechanisms in mouse macrophages.
Methods: Peritoneal macrophages from thioglycollate medium-injected mice were cultured and stimulated with
lipopolysaccharide(LPS) or LPS/interferon(IFN)-γ for cytokine measurement and cellular signaling molecule
analysis.
Results: FF ethanol extract decreased the levels of secreted tumor necrosis factor(TNF)-α and interleukin(IL)-6
in IFN-γ/LPS-stimulated cells in a concentration-dependent manner. FF extract reduced IFN-γ/LPS-induced
STAT1 phosphorylation and LPS-induced p38 and JNK activation, but not ERK1/2 activity. The extract also
inhibited LPS-induced IκBα degradation through suppression of IκBα kinase.
Conclusions: These results suggest that FF ethanol extract affects the production of TNF-α and IL-6 through
inhibition of activation of STAT-1, IκBα, p38, and JNK.
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KEYWORD
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Forsythiae Fructus, inflammation, macrophages, cytokine, signaling
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